Hydroponics8 min read

Seed Starting Trays and Seed Germination: A Grower's Guide

How to choose seed starting trays, plug trays and cell sizes by crop, which germination temperatures each vegetable needs, and how to raise uniform seedlings indoors and at commercial scale.

By Mars Vertical Farm Engineering TeamPublished

Seed Starting Trays and Seed Germination: A Grower's Guide — illustrative image

Short answer

Seed starting trays are shallow cell trays or flats used to germinate seeds and raise seedlings until transplant. Most use the 1020 footprint of about 27 × 54 cm, with plug trays of 50 to 288 cells. Lettuce typically germinates best near 24°C (75°F), tomatoes and peppers near 29°C (85°F), and cucumbers near 35°C (95°F), with seedlings then grown cooler under 14 to 16 hours of light.

What are seed starting trays?

Seed starting trays are shallow plastic or fiber containers, usually divided into cells, that hold growing medium for germinating seeds and raising seedlings until transplant. Most follow the North American 1020 footprint of about 27 × 54 cm (10.5 × 21 in), so flats, cell inserts, plug trays and humidity domes stack and fit on the same benches, shelves and carts.

For commercial growers, the tray is only one part of a seed starting system that also includes the right medium, temperature, moisture, light and watering method. This guide covers the main types of seedling trays, how to choose cell size by crop, a seed germination temperature chart, a step-by-step method for germinating seeds indoors, and how propagation scales from a seed starting shelf with lights to tidal benches and seedling cabinets. If you grow microgreens rather than transplants, see how to grow microgreens.

What types of seed starting trays and containers are there?

Seed starting trays fall into a few families, each suited to different crops and scales. The table compares the common plant trays and seed starting containers.

TypeDescriptionTypical sizesBest for
Flat tray (1020 flat)Open tray, with or without drainage holesAbout 27 × 54 cm, 5–6 cm deepCarrier for inserts, bottom watering, microgreens, sprouting trays
Cell inserts (cell packs)Thin sheets of 4- or 6-cell packs that sit in a flatOften 18 × 4-cell or 12 six-cell packs per flatBedding plants, retail, larger seedlings
Plug traysRigid one-piece trays with many tapered cells50, 72, 128, 200 and 288 cellsCommercial transplant production, hydroponic seedlings
Seed starting potsIndividual round or square pots, plastic or fiber5–10 cm (2–4 in)Potting on tomatoes, peppers, cucurbits
Biodegradable pots and blocksPeat, coir or paper pots and soil blocks planted whole4–8 cmRoot-sensitive crops, organic growers
Hydroponic plug sheetsRockwool, sponge or coir plugs in a trayOften 2–4 cm cubes, sheets of 100–200+Starting hydroponic seeds for NFT, DWC and towers
Humidity domesClear lids for seed trays, some with ventsMatched to 1020 flats, 5–18 cm tallKeeping humidity high during germination

Are reusable, heavy duty seed starting trays worth it?

Heavy duty seed starting trays made of thicker polypropylene or polystyrene last many seasons, resist cracking when lifted full of wet media, and can be washed and sanitized between crops. Thin, disposable flats are cheap but often split after one or two uses. For a nursery or greenhouse handling thousands of trays, reusable seedling trays usually cost less per crop and reduce plastic waste. Silicone seed starting trays are durable and flexible for popping plugs out, but cost more per cell and are mainly used by hobby growers.

What seed starting supplies do you need?

Beyond the trays themselves, a basic set of seed starting supplies covers most crops:

  • Trays and covers: solid and holed flats, plug or cell trays, and seed tray domes. Trays sold as a set with dome lids are the easiest way to keep humidity even.
  • Medium: a sterile seed-starting mix, or rockwool and sponge plugs for hydroponics.
  • Seedling tools: a dibber or widger, a vacuum or hand seeder for small seed, plant labels and a fine-rose watering can or mister.
  • Heat and light: a thermostat-controlled heat mat and LED lights on a timer.
  • Monitoring: a soil thermometer and a hygrometer.

The best seed starter kit for a home gardener bundles a tray, insert, dome and sometimes a heat mat and light. Growers raising thousands of plants buy plastic planting trays, nursery plant trays and plug trays by the case, along with propagation benches, so each tray handles the same way through the whole system.

How do you choose cell size for each crop?

Cell size sets how long a seedling can stay in the tray before roots run out of room, so match cells to the crop and the planned transplant age. Typical choices are:

CropTypical tray or cellTypical weeks to transplant
Lettuce and leafy greens128–200 cell plug trays or hydroponic plugs2–4 weeks
Brassicas (cabbage, broccoli, kale)72–128 cells4–6 weeks
Herb seedlings (basil, parsley, cilantro)72–128 cells, several seeds per cell3–6 weeks
Tomatoes and peppers72 cells, then pot on to 7–10 cm pots5–8 weeks
Cucumbers, squash and melons50–72 cells or 7–10 cm pots2–4 weeks
Onions and leeks128–200 cells or open flats8–10 weeks

Smaller cells such as 288 or 200 save bench space but dry out quickly and need precise watering. Bigger cells are more forgiving and give sturdier transplants.

Seed germination temperature and time chart

Seed germination temperature is the single most important factor in how fast and evenly seeds sprout. The chart below uses widely published soil temperature data for vegetable seed germination from university extension services such as the Alabama Cooperative Extension System; days to emergence are typical ranges at near-optimum temperature.

CropMinimumOptimum rangeBest temperatureMaximumTypical days to emerge
Lettuce2°C (35°F)4–27°C (40–80°F)24°C (75°F)29°C (85°F)2–7
Spinach2°C (35°F)7–24°C (45–75°F)21°C (70°F)29°C (85°F)5–10
Tomato10°C (50°F)21–35°C (70–95°F)29°C (85°F)35°C (95°F)5–10
Pepper16°C (60°F)18–35°C (65–95°F)29°C (85°F)35°C (95°F)7–14
Eggplant16°C (60°F)24–32°C (75–90°F)29°C (85°F)35°C (95°F)7–14
Cucumber16°C (60°F)16–35°C (60–95°F)35°C (95°F)41°C (105°F)3–7
Squash16°C (60°F)21–35°C (70–95°F)35°C (95°F)38°C (100°F)4–7
Cabbage4°C (40°F)7–35°C (45–95°F)29°C (85°F)38°C (100°F)4–7
Onion2°C (35°F)10–35°C (50–95°F)24°C (75°F)35°C (95°F)7–14
Parsley4°C (40°F)10–35°C (50–95°F)24°C (75°F)35°C (95°F)14–28

Two practical notes: many lettuce varieties become dormant and germinate poorly above roughly 27–29°C (80–85°F), so start lettuce seeds indoors in a cool spot in summer; and warm-season crops such as peppers and eggplant germinate slowly and unevenly below about 21°C (70°F), which is why growers use heat mats or warm germination rooms. Basil, though not in the extension table, typically germinates well at about 21–27°C (70–80°F).

How do you germinate seeds indoors?

Reliable indoor germination comes down to clean trays, a fine moist medium, the right temperature and light as soon as seedlings emerge. This method works for most vegetables and herbs:

  1. Clean the trays. Wash reused trays and soak them in a horticultural disinfectant to reduce damping-off fungi.
  2. Fill with a seed-starting mix. Use a fine, sterile peat- or coir-based mix with perlite, moistened before filling, or pre-soaked rockwool or sponge plugs for hydroponics. Do not pack it tightly.
  3. Sow at the right depth. A common rule is to sow seeds about two to three times as deep as their width. Lettuce and many herbs need light to germinate, so cover them very lightly or not at all.
  4. Water and cover. Bottom water or mist gently, then fit humidity domes to keep humidity high.
  5. Provide steady warmth. Use a germination room or a thermostat-controlled heat mat set to the crop's optimum from the chart.
  6. Remove the dome at emergence. As soon as most seedlings show, vent and then remove the dome and move trays under light, otherwise seedlings stretch within a day or two.
  7. Light and water seedlings. Give 14–16 hours of light a day, bottom water when the cells lighten in weight, and start a dilute feed (often EC 0.5–1.0 mS/cm) once true leaves appear.
  8. Harden off and transplant. Gradually expose seedlings to transplant conditions over 5–10 days before planting out or moving to hydroponic channels.

What temperature and light do seedlings need after germination?

After germination, the ideal temperature for seedlings is usually a little lower than the germination temperature: roughly 18–24°C (65–75°F) by day and a few degrees cooler at night for most vegetables, which keeps growth compact. Light intensity of about 100–300 µmol/m²/s suits most seedlings; too little light causes leggy, pale transplants. See PAR, PPFD and light spectrum for details.

When should you start herb and lettuce seeds indoors?

For outdoor planting, start lettuce seeds indoors about 3–5 weeks before transplanting, most herbs about 6–8 weeks before the last expected frost, and tomatoes and peppers about 6–10 weeks before. For year-round indoor or hydroponic production, sow on a fixed weekly schedule so a new tray is ready every week.

How do you start hydroponic seeds?

To start hydroponic seeds, sow directly into inert plugs such as rockwool, polyurethane sponge cubes or coir plugs held in a tray, so the whole plug moves into the hydroponic system without disturbing roots. Soak rockwool in water adjusted to about pH 5.5 before sowing, keep plugs moist but not waterlogged, and transplant when roots emerge from the bottom and seedlings have two to four true leaves. Lettuce in 2–3 cm sponge plugs is usually ready for NFT channels or rafts in about 2–3 weeks; our hydroponic lettuce guide covers the next stage, and the hydroponic growing media guide compares plug materials. Mars Vertical Farm supplies nursery plug sponge cubes sized for common net cups and channel holes.

How do you scale up seedling production?

Seedling production scales from shelves to benches to dedicated propagation equipment, and each step improves uniformity and labor efficiency.

  • Seed starting shelf with lights: a four- or five-tier metal rack with LED bars above each shelf holds roughly 4–8 trays per tier in a small footprint. Racks on castors double as carts for moving trays.
  • Tents and cabinets: a tent with shelving or a closed seedling cabinet adds control of temperature and humidity. Compare them in grow tent vs grow cabinet.
  • Tidal (ebb and flow) benches: greenhouse trays sit on benches that flood from below for a few minutes and then drain, watering every cell evenly and keeping leaves and the media surface drier. See ebb and flow flood tables and our customized tidal seedling tray system.
  • Rolling and multi-tier benches: rolling benches remove fixed aisles and can raise usable greenhouse area substantially, while vertical seedling tables add tiers.

What are the most common seed starting mistakes?

  • Overwatering: soggy media causes damping-off and breeds fungus gnats; see our fungus gnat control guide.
  • Leaving domes on too long: causes stretching and disease.
  • Too little light: a sunny windowsill rarely provides enough for compact seedlings.
  • Wrong temperature: too cold slows germination; too hot stops lettuce and spinach from germinating.
  • Old or poorly stored seed: run a germination test on a damp paper towel before sowing large batches.

Next steps

The right seed starting trays, matched to crop, cell size and a well-controlled germination environment, are the foundation of uniform transplants and reliable harvests. If you are planning a nursery, greenhouse propagation area or seedling room, tell us your crops, weekly tray count and space and we can propose factory-direct tidal benches, seedling cabinets and plug systems in standard or custom sizes.

Mars Vertical Farm Engineering Team

Systems engineering, Shanghai. Written by the engineers and project managers who design and ship Mars Vertical Farm systems. Ask the author

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What is the best seed starting tray?

For most growers, a heavy duty 1020 flat with a matching plug tray or cell insert and a humidity dome is the most versatile choice. Choose 128 to 200 cells for lettuce and leafy greens and 50 to 72 cells for tomatoes, peppers and cucurbits. Thicker reusable trays last many seasons and can be sanitized between crops.

What temperature is best for germinating seeds?

It depends on the crop. Lettuce and spinach germinate best around 21 to 24 degrees Celsius (70 to 75°F), tomatoes, peppers and eggplant around 29°C (85°F), and cucumbers and squash around 35°C (95°F). Many lettuce varieties germinate poorly above about 27 to 29°C, while peppers are slow below about 21°C.

How long do seeds take to germinate?

At near-optimum temperatures, lettuce and cucumbers often emerge in 2 to 7 days, tomatoes in 5 to 10 days, peppers and eggplant in 7 to 14 days, and parsley in 2 to 4 weeks. Cooler media, old seed or uneven moisture can double these times, which is why growers use heat mats or germination rooms.

Should seed trays have holes?

Cell trays and plug trays should have drainage holes so excess water can escape and the trays can be bottom watered. Many growers set them inside a solid flat with no holes to hold water for bottom watering. Holed flats are used on benches, capillary mats and tidal or ebb and flow tables.

When should I remove the dome from my seed tray?

Remove or vent the dome as soon as most seeds have emerged, usually within a day of the first seedlings appearing. Leaving the dome on traps humidity and heat, which causes stretched, weak seedlings and raises the risk of damping-off. Move trays under light at the same time.

How do you start seeds for hydroponics?

Sow seeds directly into inert plugs such as rockwool, sponge cubes or coir plugs held in a tray. Soak rockwool in water adjusted to about pH 5.5, keep plugs moist but not waterlogged, and provide light as soon as seedlings emerge. Transplant into the hydroponic system when roots show through the bottom of the plug.

How many weeks before transplanting should I start seeds?

Lettuce and leafy greens are usually ready 2 to 5 weeks after sowing, brassicas 4 to 6 weeks, most herbs 3 to 8 weeks, tomatoes and peppers 5 to 10 weeks, and cucurbits only 2 to 4 weeks because they dislike root disturbance. For outdoor planting, count back from your last expected frost date.

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